Journal: Applications of Nanomaterials in Human Health

Article Title: Nanomaterials and Their Negative Effects on Human Health

doi: 10.1007/978-981-15-4802-4_13

Figure Lengend Snippet: G5 PAMAM dendrimers toxicity in lung (Sun et al. )

Article Snippet: 1. , Diaminobutane core (DAB) based generation 3, 4, and 5 dendrimer (diaminobutyric polypropylenimine) for gene delivery , Diaminobutyric polypropylenimine (3-, 4-, and 5-), methoxy PEG (~2 kDa) succinimidyl carboxymethyl esters , Bioware PC-3 M-luc-C6 human prostate adenocarcinoma, bioware B16F10-Luc cells, A431 human epidermoid carcinoma, T98G human glioblastoma, DU145 human prostate carcinoma , – , PEGylated G3 and G4-DAB reduced cytotoxicity of dendrimer. G4-DAB with PEG (2 and 5 kDa) reduced toxicity of dendrimer at lower dose of 20 μg/ml , Somani et al. ( ) .

Techniques:

Journal: Applications of Nanomaterials in Human Health

Article Title: Nanomaterials and Their Negative Effects on Human Health

doi: 10.1007/978-981-15-4802-4_13

Figure Lengend Snippet: Impact of nanomaterials on organs/cells/tissues

Article Snippet: 1. , Diaminobutane core (DAB) based generation 3, 4, and 5 dendrimer (diaminobutyric polypropylenimine) for gene delivery , Diaminobutyric polypropylenimine (3-, 4-, and 5-), methoxy PEG (~2 kDa) succinimidyl carboxymethyl esters , Bioware PC-3 M-luc-C6 human prostate adenocarcinoma, bioware B16F10-Luc cells, A431 human epidermoid carcinoma, T98G human glioblastoma, DU145 human prostate carcinoma , – , PEGylated G3 and G4-DAB reduced cytotoxicity of dendrimer. G4-DAB with PEG (2 and 5 kDa) reduced toxicity of dendrimer at lower dose of 20 μg/ml , Somani et al. ( ) .

Techniques: Solvent, In Vitro, In Vivo, Synthesized, Animal Model, Concentration Assay, Activity Assay, Expressing, Blocking Assay, Titanium Dioxide

Journal: Pharmaceutics

Article Title: Evolution from Covalent to Self-Assembled PAMAM-Based Dendrimers as Nanovectors for siRNA Delivery in Cancer by Coupled In Silico-Experimental Studies. Part I: Covalent siRNA Nanocarriers

doi: 10.3390/pharmaceutics11070351

Figure Lengend Snippet: Equilibrated molecular dynamics conformations of G 5 TEA-core ( a ) and NH 3 -core ( b ) poly(amidoamine) (PAMAM) dendrimers in physiological solution (pH = 7.4, ionic strength = 0.15 M NaCl). Each dendrimer molecule is represented as colored sticks, some ions and counterions are visualized as purple (Na + ) and green (Cl − ) spheres, and water molecules are not shown for clarity. Average radial monomer density ρ ( r ) for subsequent generations (from G 0 to G 5 ) of the TEA-core ( c ) and the NH 3 -core PAMAMs ( d ). In all cases, the origin was set at the molecular center of mass. Adapted from , published by RSC, 2013.

Article Snippet: The results from all in silico investigations described above unambiguously supported the conclusion that, among all ssiRNAs with non-complementary overhangs we synthesized to empower low-generation TEA-core PAMAMs with effective delivery capacity, those bearing A 7 /T 7 terminals represent the best compromise in terms of both nanovector binding and unbinding ability.

Techniques:

Journal: Pharmaceutics

Article Title: Evolution from Covalent to Self-Assembled PAMAM-Based Dendrimers as Nanovectors for siRNA Delivery in Cancer by Coupled In Silico-Experimental Studies. Part I: Covalent siRNA Nanocarriers

doi: 10.3390/pharmaceutics11070351

Figure Lengend Snippet: In silico normalized free energy of binding (ΔG bind / N ) and its major components (binding enthalpy ΔH bind / N and entropy variation –TΔS bind / N ) for G 4 –G 6 TEA-core and NH 3 -core PAMAMs in complex with heat shock protein 27 (Hsp27) small interfering RNA (siRNA) at pH 7.4 and 0.15 M NaCl. The normalization factor N is the generation-specific total number of charged dendrimer terminal groups ( N ). All values are expressed in kcal/mol 1 . Adapted from [ 35 ] with permission of John Wiley and Sons.

Article Snippet: The results from all in silico investigations described above unambiguously supported the conclusion that, among all ssiRNAs with non-complementary overhangs we synthesized to empower low-generation TEA-core PAMAMs with effective delivery capacity, those bearing A 7 /T 7 terminals represent the best compromise in terms of both nanovector binding and unbinding ability.

Techniques: In Silico, Binding Assay, Small Interfering RNA

Journal: Pharmaceutics

Article Title: Evolution from Covalent to Self-Assembled PAMAM-Based Dendrimers as Nanovectors for siRNA Delivery in Cancer by Coupled In Silico-Experimental Studies. Part I: Covalent siRNA Nanocarriers

doi: 10.3390/pharmaceutics11070351

Figure Lengend Snippet: Equilibrated molecular dynamics conformations of G 5 TEA-core ( a ) and NH 3 -core ( b ) PAMAM dendrimers in complex with Hsp27 siRNA in physiological solution (pH = 7.4, ionic strength = 0.15 M NaCl). Each dendrimer molecule is represented as colored sticks, some ions and counterions are visualized as light pink (Na + ) and light green (Cl − ) spheres, the siRNAs are portrayed as light blue ribbons, and water molecules are not shown for clarity. Radial density distribution ρ ( r ) of the dendrimer terminal nitrogen atoms in G 5 TEA-core ( c ) and NH3-core ( d ) PAMAMs in complex with Hsp27 siRNA (continuous lines). The corresponding distributions of the siRNA phosphorous atoms in each siRNA/dendrimer complex are shown as dashed lines. Adapted from [ , ] with permission of John Wiley and Sons and Bentham Science Publishers.

Article Snippet: The results from all in silico investigations described above unambiguously supported the conclusion that, among all ssiRNAs with non-complementary overhangs we synthesized to empower low-generation TEA-core PAMAMs with effective delivery capacity, those bearing A 7 /T 7 terminals represent the best compromise in terms of both nanovector binding and unbinding ability.

Techniques:

Journal: Pharmaceutics

Article Title: Evolution from Covalent to Self-Assembled PAMAM-Based Dendrimers as Nanovectors for siRNA Delivery in Cancer by Coupled In Silico-Experimental Studies. Part I: Covalent siRNA Nanocarriers

doi: 10.3390/pharmaceutics11070351

Figure Lengend Snippet: Three-dimensional atomic force microscopy (AFM) images of ( a ) Hsp27 siRNA in complex with TEA-core PAMAM dendrimers of increasing generation ( G 1 – G 7 ) and ( b ) a single spherical siRNA/TEA-core dendrimer ( G 7) complex at a final siRNA concentration of 0.0125 mg/L and at a dendrimer-to-siRNA charge ratio (N/P) of 10. ( c ) Gel retardation of Hsp27 siRNA with three different TEA-core PAMAMs (( G 1 ) ( a ), ( G 4 ) ( b ) and ( G 7 ) ( c )) as a function of the N/P ratio (from 10/1 to 1/10 from left to right; last lane: Naked siRNA). Adapted from with the permission of the RSC.

Article Snippet: The results from all in silico investigations described above unambiguously supported the conclusion that, among all ssiRNAs with non-complementary overhangs we synthesized to empower low-generation TEA-core PAMAMs with effective delivery capacity, those bearing A 7 /T 7 terminals represent the best compromise in terms of both nanovector binding and unbinding ability.

Techniques: Microscopy, Concentration Assay, Electrophoretic Mobility Shift Assay

Journal: Pharmaceutics

Article Title: Evolution from Covalent to Self-Assembled PAMAM-Based Dendrimers as Nanovectors for siRNA Delivery in Cancer by Coupled In Silico-Experimental Studies. Part I: Covalent siRNA Nanocarriers

doi: 10.3390/pharmaceutics11070351

Figure Lengend Snippet: ( a ) Hsp27 gene silencing upon delivery of complementary ssiRNAs mediated by different generations of TEA-core PMAMAM to PC-3 cells. Checked bars: Data for A 5 /T 5 ssiRNA; solid bars: Data for A 7 /T 7 ssiRNA. In these experiments, vinculin was used as reference and non-treated cells were used for control. ( b ) Long-term Hsp27 silencing achieved with A 5 /T 5 and A 7 /T 7 ssiRNAs (50 nM) delivered by G 5 TEA-core PAMAMs at N/P = 10. Redrawn from , with permission of the American Chemical Society.

Article Snippet: The results from all in silico investigations described above unambiguously supported the conclusion that, among all ssiRNAs with non-complementary overhangs we synthesized to empower low-generation TEA-core PAMAMs with effective delivery capacity, those bearing A 7 /T 7 terminals represent the best compromise in terms of both nanovector binding and unbinding ability.

Techniques: Control